OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments
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چکیده
منابع مشابه
OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments
Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method. The method takes advantage of both homologous recombination enzymes in E. coli and overlap PCR. ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/srep44648